Intermediate

Part:BBa_K273004:Design

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-16)

RBS - Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1033
    Illegal AgeI site found at 1180
    Illegal AgeI site found at 1558
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 67
    Illegal SapI site found at 481
    Illegal SapI site found at 1144


Design Notes

none


Source

Source of the alcohol deyhydrogenase 2 (adh2) is Z. Mobilis ATCC 29191 via Phusion PCR with the following primers:

pdc_forward CGCgaattcgcggccgcttctagATGAGTTATACTGTCGGTACC 44nt

pdc_reverse CGctgcagcggccgctactagtaTTATTAGAGGAGCTTGTTAACAGGC 48nt


The strain was a kind gift of [http://www.fz-juelich.de/ibt/physio Microbial Physiology Group FZ Jülich, Dr. Stephanie Bringer]


BioBricked by the Uppsala iGEM Team 2009

References